BioCentriq is announcing the release of a white paper titled Efficient, Large-Scale Transfection of T-Cells Using Flowfect® Technology.
Transfection is an invaluable tool used in cell engineering applications to study and modify gene expression by introducing mRNA or DNA into a recipient cell.
Traditional transfection methods, such as static electroporation, typically result in adverse effects to cell viability, delivery efficiency, and subsequent cell growth. Kytopen’s Flowfect®Tx is an automated, closed, benchtop transfection system that enables the delivery of genetic payloads to cells through electromechanical continuous flow.
The objective of the study, conducted through a collaboration between BioCentriq and Kytopen, was to test transfection of T-cells with GFP mRNA for evaluation of cell viability, transfection efficiency, and posttransfection growth using Kytopen’s Flowfect® technology. GFP-labeled cells were analyzed using flow cytometry methods as a measure of transfection efficiency.
Request the full white paper by completing the form below and it will be sent to you in email. If you have any additional questions, please contact us.